Open AccessRole of gyrA mutation and loss of OprF in the multiple antibiotic resistance phenotype of Pseudomonas aeruginosa G49
Author(s) -
Pumbwe Lilian,
Everett Martin J.,
Hancock Robert E.W.,
Piddock Laura J.V.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08456.x
Subject(s) - pseudomonas aeruginosa , phenotype , microbiology and biotechnology , pseudomonadales , mutation , pseudomonadaceae , genetics , biology , antibiotic resistance , antibiotics , bacteria , gene
A clinical isolate of Pseudomonas aeruginosa G48, became resistant during fluoroquinolone treatment giving rise to the post‐therapy isolate, G49. To determine whether mutation in gyrA gave rise to fluoroquinolone resistance, G49 was transformed with a plasmid encoding gyrA (pNJR3‐2); this reduced the MIC of fluoroquinolones for G49 two‐fold. DNA sequencing of gyrA of G49 demonstrated a mutation at Thr‐83, substituting with isoleucine. The outer membrane of G49 was shown to lack OprF, suggesting that loss of this protein may be involved in the multiple antibiotic resistance phenotype; however, when G49 was transformed with a plasmid encoding oprF (pRWS), expression of oprF was shown to have no effect upon the phenotype.