Open AccessAnalysis of sodium dodecyl sulfate‐stable cell wall aminopeptidases in strains of viridans streptococci
Author(s) -
Linder Lars E.,
Lönnies Helena,
Sund Marielouise
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08455.x
Subject(s) - microbiology and biotechnology , aminopeptidase , sodium dodecyl sulfate , gel electrophoresis , biology , bacteria , streptococcus sobrinus , polyacrylamide gel electrophoresis , streptococcaceae , cell wall , streptococcus , biochemistry , enzyme , chemistry , leucine , amino acid , streptococcus mutans , antibiotics , genetics
Cell wall extracts from nine strains of viridans streptococci representing five species were analyzed for aminopeptidases. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis in combination with zymogram procedures revealed a common pattern of aminopeptidases in six strains, all having enzyme bands at 89 and 45 kDa. Three strains had an additional band at 200 kDa. Crossreactivity between aminopeptidases of all active strains was shown with crossed immunoelectrophoresis. Strains of Streptococcus mutans and S. sobrinus were without detectable cell wall aminopeptidases.