Open AccessCloning and sequencing of the novel abortive infection gene abiH of Lactococcus lactis ssp. lactis biovar. diacetylactis S94
Author(s) -
Prévots Fabien,
Daloyau Marlène,
Bonin Odile,
Dumont Xavier,
Tolou Sandrine
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08446.x
Subject(s) - biovar , lactococcus lactis , homology (biology) , biology , gene , bacteriophage , cloning (programming) , genetics , microbiology and biotechnology , molecular cloning , bacteria , peptide sequence , escherichia coli , lactic acid , computer science , programming language
A gene which encodes resistance by abortive infection (Abi + ) to bacteriophage was cloned from Lactococcus lactis ssp. lactis biovar. diacetylactis S94. This gene was found to confer a reduction in efficiency of plating and plaque size for prolate‐headed bacteriophage φ53 (group I of homology) and total resistance to the small isometric‐headed bacteriophage φ59 (group III of homology). The cloned gene is predicted to encode a polypeptide of 346 amino acid residues with a deduced molecular mass of 41 455 Da. No homology with any previously described genes was found. A probe was used to determine the presence of this gene in two strains on 31 tested.