Open AccessIdentification of a multigene family coding for the 90 kDa proteins of the ovine abortion subtype of Chlamydia psittaci
Author(s) -
Longbottom David,
Russell Mary,
Jones Gareth E.,
Lainson F. Alexander,
Herring Alan J.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08443.x
Subject(s) - chlamydia psittaci , biology , gene , chlamydiae , genomic dna , chlamydiaceae , southern blot , genetics , antigen , virology , chlamydia , microbiology and biotechnology
While attempting to identify genes and their corresponding antigens that could be used to improve the current methods of diagnosing Chlamydia psittaci infection which causes enzootic abortion in ewes, two candidate clones were isolated from a λgt11 genomic DNA expression library of ovine abortion subtype (strain S26/3) C. psittaci . These clones contained fragments of a gene coding for a group of three chlamydial proteins of approximately 90 kDa which appeared as major immunogens by immunoblotting experiments, indicating their potential as diagnostic or possibly protective antigens. Southern blotting of S26/3 genomic DNA using the two clones as probes identified a family of three or four genes. These represent the first example of protein gene duplication reported in Chlamydia .