Open AccessExpanded genomic map of Campylobacter jejuni UA580 and localization of 23S ribosomal rRNA genes by I‐ CeuI restriction endonuclease digestion
Author(s) -
Newnham Elizabeth,
Chang Nicholas,
Taylor Diane E.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08434.x
Subject(s) - 23s ribosomal rna , biology , campylobacter jejuni , genetics , ribosomal rna , restriction enzyme , gene , campylobacter , restriction map , pulsed field gel electrophoresis , microbiology and biotechnology , plasmid , rna , bacteria , ribosome , genotype
The genomic map of Campylobacter jejuni UA580 was expanded and more precisely constructed using I‐ Ceu I, Sal I and Sma I restriction endonucleases in conjunction with pulsed‐field gel electrophoresis (PFGE). The presence of three fragments after digestion with I‐ Ceu I confirmed the presence of three copies of the 23S ribosomal rRNA ( rrl ) gene. The genome size of Campylobacter jejuni UA580 was determined to be 1725 ± 5.9 kbp by I‐CeuI with fragment sizes of 1053 ± 4.4, 361 ± 2.7 and 311 ± 3.6 kbp. Analysis of a PCR product from C. jejuni UA580 23S rRNA gene showed that I‐ Ceu I did cut within the gene. The precise locations of the three genes were determined using I‐ Ceu I with two copies of the 23S and 5S rRNA genes located separately from the 16S rRNA gene whereas the third copy of the 23S and 5S rRNA genes had a closer linkage to a 16S rRNA gene copy. Homologous gene probes were used to map additional genes and allowed the realignment of a few previously mapped genes on the chromosome. Other strains of C. jejuni were also cut into three fragments with I‐ Ceu I, which generated variable PFGE patterns.