Open AccessThe use of a new viability assay to determine the susceptibility of Cryptosporidium and Eimeria sporozoites to respiratory inhibitors and extremes of pH
Author(s) -
Brown Samantha M.A.,
McDonald Vincent,
Denton Helen,
Coombs Graham H.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08431.x
Subject(s) - cryptosporidium parvum , acridine orange , eimeria , biology , cryptosporidium , viability assay , microbiology and biotechnology , sodium azide , apicomplexa , cyanide , parasite hosting , biochemistry , chemistry , cell , malaria , immunology , protozoal disease , feces , inorganic chemistry , apoptosis , world wide web , computer science
A new viability assay for Cryptosporidium and Eimeria sporozoites is described. It involves the use of both acridine orange and bis‐benzimide and is more rapid, easier and less subjective than procedures used previously. The assay has been used to investigate the effects of respiratory inhibitors and pH on the sporozoites of C. parvum, C. muris and E. tenella . Neither cyanide nor azide reduced the viability of C. parvum or E. tenella , whereas they had some effect on C. muris . This latter organism, an intracellular parasite of stomach epithelial cells, also differed from the other two in being able to survive pH 2 for as long as 1 h.