Open AccessIsolation and initial characterization of aerobic chloromethane‐utilizing bacteria
Author(s) -
Doroni.V.,
Sokolov A.P.,
Trotsenko Y.A.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08427.x
Subject(s) - chloromethane , bacteria , methylobacterium , chemistry , isolation (microbiology) , methylotroph , biochemistry , energy source , formaldehyde , microbiology and biotechnology , formate , enzyme , biology , catalysis , organic chemistry , genetics , natural gas , 16s ribosomal rna
Eight strains of non‐methane‐utilizing aerobic methylotrophic bacteria able to grow on chloromethane as the carbon and energy source have been isolated. Based on their phenotypic and genomic characteristics the new isolates were classified as Hyphomicrobium spp. (strains CM1, CM2, CM9, CM29, CM35) and Methylobacterium spp. (strains CM4, CM30, CM34). All the strains possessed an inducible yet unknown enzyme that catalyzed conversion of chloromethane to HCl and formaldehyde. The latter was oxidized via formate to CO 2 or assimilated through icl + or icl − variants of the serine pathway.