Open AccessD‐cycloserine biases enrichment for auxotrophic mutants of Zymomonas mobilis
Author(s) -
Pencreac'h Gaelic,
AïtAbdelkader Nadra,
Joset Françoise,
Baratti Jacques C.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08425.x
Subject(s) - zymomonas mobilis , cycloserine , mutant , glycine , chemistry , auxotrophy , methionine , glutamic acid , bacteria , biochemistry , microbiology and biotechnology , biology , fermentation , amino acid , antibiotics , gene , genetics , ethanol fuel
Contrary to its effect on rich medium, d‐cycloserine showed no bactericidal effect on Zymomonas mobilis cells cultured on mineral medium. Addition of a mixture of glycine and glutamic acid to the mineral medium restored its bactericidal action. However, mutant enrichments run in these conditions were biased, with mostly methionine mutants isolated. A decrease of the d‐cycloserine concentration only reduced the bias.