Open AccessPCR‐based restriction fragment length polymorphism (RFLP) analysis and serotyping of Campylobacter jejuni isolates from diarrheic patients in China and Japan
Author(s) -
Nishimura Masataka,
Nukina Masafumi,
Yuan Jin Mei,
Shen Bao Quan,
Ma Jian Jun,
Ohta Mitsuhiro,
Saida Takahiko,
Uchiyama Takashi
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08420.x
Subject(s) - restriction fragment length polymorphism , typing , biology , campylobacter jejuni , serotype , restriction enzyme , microbiology and biotechnology , flagellin , molecular epidemiology , haeiii , virology , genotype , genetics , gene , bacteria
A molecular typing approach for Campylobacter jejuni was applied with restriction fragment length polymorphism (RFLP) analysis of a 702‐bp PCR‐amplified portion of the flagellin‐A ( flaA ) gene. We analyzed a total of 179 strains, including 69 independent clinical isolates from diarrheic patients in Japan, 85 isolates in China, and 25 heat‐stable (HS) serotype strains by Penner and Hennessy ((1980) J. Clin. Microbiol. 12, 732–737). Six Afa I, seven Mbo I, and five Hae III RFLPs were found in the 702‐bp flaA segment from the 179 strains. Using a combination of these three enzymes, 25 separate RFLP groups were recognized. While 59 of 154 (38.3%) strains obtained in Japan and China were nontypeable by the HS antigenic scheme, all but two of 154 (98.7%) could be typed by RFLP typing. All 11 isolates of HS‐19 strains, which are frequently isolated from Guillain‐Barré syndrome (GBS) patients, showed an identical RFLP pattern (Cj‐1), and Cj‐1 consisted only of HS‐19 strains. This suggests that the HS‐19:Cj‐l strain is distinct among C. jejuni strains. This molecular typing method provides a rapid and reliable typing scheme for epidemiological studies of C. jejuni , and may also be useful for the analysis of C. jejuni subtypes from GBS patients.