Open AccessComplete development of Cryptosporidium parvum in MDBK cells
Author(s) -
Villacorta Isabel,
Graaf Dirk,
Charlier Gerard,
Peeters Johan E.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08419.x
Subject(s) - cryptosporidium parvum , microbiology and biotechnology , biology , in vitro , parasite hosting , immunofluorescence , cryptosporidium , virology , antibody , immunology , biochemistry , world wide web , computer science , feces
Sporozoites of Cryptosporidium parvum excysted in vitro from bovine oocysts were incubated with monolayers of Madin‐Darby bovine kidney cells. The extent of parasite colonisation was monitored by light microscopy and immunofluorescence. Electron microscopy confirmed the complete development and replication of C. parvum within Madin‐Darby bovine kidney cells.