Open Access16S‐23S ribosomal RNA spacer regions of Acetobacter europaeus and A. xylinum , tRNA genes and antitermination sequences
Author(s) -
Sievers Martin,
Alonso Lorenzo,
Gianotti Sabine,
Boesch Cornelia,
Teuber Michael
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08405.x
Subject(s) - spacer dna , biology , 23s ribosomal rna , internal transcribed spacer , ribosomal rna , transfer rna , nucleic acid sequence , genetics , antitermination , microbiology and biotechnology , gene , rna , ribosome , rna polymerase
The 16S‐23S ribosomal RNA spacer regions of Acetobacter europaeus DSM 6160, A. xylinum NCIB 11664 and A. xyUnion CL27 were amplified by PCR. Specific PCR products were obtained from each strain and their nucleotide sequences determined. The spacer region of A. europaeus comprises 768 nucleotides (nt), that of A. xylinum 778 nt and that of A. xylinum CL27 759 nt. Genes encoding tRNA Ile and tRNA Ala were identified. Putative antitermination sequences were found between the tRNA Ala sequence and the 5′‐terminus of the 23S rRNA coding sequence. The boxA element has the nucleotide sequence TGCTCTTTGATA. Based on hybridization data of digested chromosomal DNA with spacer‐specific probes, the copy number of the rrn operons on the chromosome of Acetobacter strains is estimated to be four.