Open AccessInfluence of the 20‐kDa protein from Bacillus thuringiensis ssp. israelensis on the rate of production of truncated Cry1C proteins
Author(s) -
Rang Cécile,
Bes Martine,
LullienPellerin Valérie,
Wu Dong,
Federici Brian A.,
Frutos Roger
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08395.x
Subject(s) - bacillus thuringiensis , bacillaceae , biology , bacillales , inclusion bodies , recombinant dna , gene , microbiology and biotechnology , bacteria , biochemistry , genetics , bacillus subtilis
The potential role of a molecular chaperone on the rate of production of extensively altered Bacillus thuringiensis Cry 1C proteins was investigated. Analysis of the proteins produced by the recombinant B. thuringiensis strains showed that the truncated proteins were produced at a low rate. Expression of the 20‐kDa protein gene from B. thuringiensis ssp. israelensis in tandem with the truncated‐ cry1C genes led to the production of a greater amount of proteins. The formation of inclusion bodies, however, did not occur even when the 20‐kDa protein gene was expressed.