Open AccessEnhancement and repression of cyanide‐insensitive respiration in Aspergillus niger
Author(s) -
Kirimura Kohtaro,
Matsui Tomoko,
Sugano Shuji,
Usami Shoji
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08393.x
Subject(s) - antimycin a , aspergillus niger , cycloheximide , salicylhydroxamic acid , alternative oxidase , biochemistry , cyanide , puromycin , protein biosynthesis , respiration , cytosol , oxidase test , biology , mitochondrion , glucose oxidase , chemistry , enzyme , organic chemistry , botany
The biosynthesis of the alternative oxidase protein consisting of cyanide‐insensitive and salicylhydroxamic acid‐sensitive respiration in Aspergillus niger was investigated. Cyanide‐insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide‐ m ‐chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.