Open AccessStructural studies on the Escherichia coli O101 lipopolysaccharide found in association with F41 and K99 fimbriae
Author(s) -
Karamanos Yannis,
Kol Ossarath
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08388.x
Subject(s) - heptose , lipopolysaccharide , escherichia coli , galactose , enterobacteriaceae , oligosaccharide , biochemistry , mannose , chemistry , microbiology and biotechnology , biology , enterotoxigenic escherichia coli , mutant , gene , endocrinology , enterotoxin
In this study it was shown that the O101 lipopolysaccharide isolated from Escherichia coli B41 did not contain an O‐specific polysaccharide and that its sugar moiety is probably restricted to the core oligosaccharide. It is characterized by the presence of galactose, glucose, N ‐acetylglucosamine, heptose and 3‐deoxy‐d‐ manno ‐2‐octulosonic acid and the fatty acid composition is typical of an Enterobacteriaceae lipopolysaccharide. Methylation analysis indicated terminal non‐reducing galactose and glucose and also 1,2‐linked glucose which is a substitution pattern typical of an E. coli lipopolysaccharide core oligosaccharide. The obtained structural information is sufficient to explain the previously observed interactions between the O101 lipopolysaccharide and the K99 lectin.