Open AccessTargeted mutation of the SC3 hydrophobin gene of Schizophyllum commune affects formation of aerial hyphae
Author(s) -
Wetter MarieAnne,
Schuren Frank H.J.,
Schuurs Theo A.,
Wessels Joseph G.H.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08347.x
Subject(s) - schizophyllum commune , hydrophobin , hypha , complementation , mutant , dikaryon , microbiology and biotechnology , biology , basidiomycota , clone (java method) , gene , chemistry , genetics , biochemistry , botany
The SC3 hydrophobin gene of Schizophyllum commune was disrupted by homologous integration of an SC3 genomic fragment interrupted by a phleomycin resistance cassette. The phenotype of the mutant was particularly clear in sealed plates in which formation of aerial hyphae was blocked. In non‐sealed plates aerial hyphae did form but these were hydrophilic and not hydrophobic as in wild‐type strains. Complementation with a genomic SC3 clone restored formation of hydrophobic aerial hyphae in sealed plates. In a dikaryon homozygous for the SC3 mutation normal sporulating fruiting bodies were produced but aerial hyphae were hydrophilic.