Open AccessImproved electro‐transformation of highly DNA‐restrictive corynebacteria with DNA extracted from starved Escherichia coli
Author(s) -
Ankri Serge,
Reyes Oscar,
Leblon Gérard
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08344.x
Subject(s) - escherichia coli , transformation (genetics) , dna , microbiology and biotechnology , chemistry , escherichia coli proteins , biology , genetics , gene
Differences of up to 33000‐fold in electro‐transformability of highly DNA restrictive corynebacteria are observed in the DNA of a shuttle plasmid extracted from Escherichia coli hosts propagated in different nutritional conditions. Growth of the host in minimal medium increases plasmid transformability, whereas growth on rich media decreases it. In the E. coli DH5a host, the starvation‐dependent increase in DNA transformability is reverted by supplementing with methionine, an obligate S‐adenosyl‐methionine (SAM) precursor. This suggests that an E. coli nutritionally modulated SAM‐dependent DNA‐methyltransferase may be involved in this phenomenon.