Open AccessMolecular characterization of Lactobacillus casei strains
Author(s) -
Ferrero M.,
Cesena C.,
Morelli L.,
Scolari G.,
Vescovo M.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08339.x
Subject(s) - lactobacillus casei , lactobacillus paracasei , pulsed field gel electrophoresis , biology , 23s ribosomal rna , ribosomal rna , microbiology and biotechnology , homogeneous , lactobacillaceae , gel electrophoresis , gene , lactobacillus , bacteria , genetics , rna , ribosome , genotype , physics , thermodynamics
Three different molecular techniques were used to characterize bacterial strains isolated from dairy products and phenotypically classified as Lactobacillus casei . Some strains were taxonomically identified as Lb. casei and Lb. paracasei using 23S rRNA‐targeted probes specific for Lb. casei and Lb. paracasei . Chromosomal DNA was analyzed by pulsed‐field gel electrophoresis. Lb. paracasei strains had a variable number of Sfi I fragments harboring rRNA genes, while Lb. casei strains showed more homogeneous patterns. The chromosome size of Lb. paracasei ATCC 334 was evaluated to be 2.17 Mb.