Open AccessThe effect of the length of a malarial epitope on its antigenicity and immunogenicity in an epitope presentation system using the Pseudomonas aeruginosa outer membrane protein OprF as the carrier
Author(s) -
Wong Rebecca S.Y.,
Hancock Robert E.W.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08338.x
Subject(s) - epitope , antigenicity , immunogenicity , bacterial outer membrane , virology , linear epitope , biology , epitope mapping , microbiology and biotechnology , antigen , chemistry , biochemistry , immunology , escherichia coli , gene
This study showed that the antigenicity of a malarial epitope increased with the length of the epitope when inserted at positions aa 26 (amino acid position 26) and aa 196 , but not at aa 213 , of the Pseudomonas aeruginosa major outer membrane protein OprF (326 amino acids). Immunization studies showed that a 19‐aa epitope was significantly more immunogenic than a 7‐aa epitope when inserted at aa 26 of OprF, while neither an 11 ‐ nor a 19‐aa epitope fused to the C‐terminus of glutathione 5‐transferase was immunogenic.