Open AccessExpression of Dictyostelium early gene, dutA , is independent of cAMP pulses but dependent on protein kinase A
Author(s) -
Kumimoto Hiroshi,
Yoshida Hiderou,
Okamoto Koji
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08324.x
Subject(s) - dictyostelium discoideum , mycetozoa , gene , dictyostelium , protein kinase a , protein subunit , biology , gene expression , kinase , mutant , microbiology and biotechnology , genetics
The untranslatable, RNA polymerase II‐dependent gene ( dutA ) of Dictyostelium discoideum is induced early in development. However, unlike other early genes, dutA induction was not affected by cAMP pulses and occurred normally in various cAMP‐related mutant cells, the results indicating that this induction depended solely on factors other than cAMP. In the knockout strain of the catalytic subunit of protein kinase A, dutA expression was severely blocked and not recovered by cAMP pulses. This demonstrates that even the cAMP‐independent gene, dutA , requires protein kinase A for its expression.