Open AccessIsolation and characterization of pLS 1 plasmid mutants with increased copy numbers
Author(s) -
Acebo Paloma,
Alda María Teresa,
Espinosa Manuel,
Del Solar Gloria
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08319.x
Subject(s) - plasmid , replicon , mutant , biology , genetics , phenotype , allele , gene , plasmid preparation , isolation (microbiology) , microbiology and biotechnology , pbr322
Streptococcus pneumoniae genetic systems designed for isolation of plasmid mutants with copy‐up phenotypes have been developed. The target plasmids have the pLS1 replicon, and two different strategies have been followed: (i) selection of clones exhibiting augmented resistance to antibiotics, or (ii) obligatory co‐existence of incompatible plasmids. We have isolated 23 plasmid mutants exhibiting increased number of copies. All the mutations corresponded to four different alleles of the copG gene of plasmid pLS1. These strategies could be used with other plasmids.