Open AccessThe pell mutant of Saccharomyces cerevisiae is deficient in cardiolipin and does not survive the disruption of the CHO1 gene encoding phosphatidylserine synthase
Author(s) -
Janitor Martin,
Obernauerová Margita,
Kohlwein Sepp D,
Šubík Július
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08312.x
Subject(s) - cardiolipin , mutant , phosphatidylserine , saccharomyces cerevisiae , biology , gene , biochemistry , phosphatidylinositol , serine , phosphatidylcholine , yeast , microbiology and biotechnology , enzyme , phospholipid , signal transduction , membrane
Cells of the pell mutant of Saccharomyces cerevisiae were found to contain an extremely low content of cardiolipin, a decreased level of phosphatidylcholine and an increased level of phosphatidylinositol. Disruption of the PELL gene in cells containing a null mutation in the CHO1 gene was lethal. Despite its putative functional homology with CHO1 , the overexpression of the PELL gene in the chol null mutant did not restore the wild‐type properties of the transformed cells and failed to stimulate the incorporation of l‐[3‐ 3 H]serine into total lipids of the intact yeast cells.