Open AccessCharacterisation of the Aspergillus nidulans fr A1 mutant: hexose phosphorylation and apparent lack of involvement of hexokinase in glucose repression
Author(s) -
Ruijter George J.G.,
Panneman Henk,
Den Broeck Hetty C.,
Bennett James M.,
Visser Jaap
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08206.x
Subject(s) - aspergillus nidulans , hexokinase , mutant , complementation , hexose , biochemistry , psychological repression , glucokinase , biology , fructose , phosphorylation , glycolysis , enzyme , gene , gene expression
Hexose phosphorylation was studied in Aspergillus nidulans wild‐type and in a fructose non‐utilising mutant ( frA ). The data indicate the presence of at least one hexokinase and one glucokinase in wild‐type A. nidulans , while the fr A1 mutant lacks hexokinase activity. The A. nidulans gene encoding hexokinase was isolated by complementation of the fr A1 mutation. The absence of hexokinase activity in the fr A1 mutant did not interfere with glucose repression of the enzymes involved in alcohol and l‐arabinose catabolism. This suggests that, unlike the situation in yeast where mutation of hexokinase PII abolishes glucose repression, the A. nidulans hexokinase might not be involved in glucose repression.