Open AccessIdentification of a developmentally regulated Gα protein in Blastocladiella emersonii
Author(s) -
Terenzi Hernán,
Da Costa Maia JoséCarlo
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08182.x
Subject(s) - adenylyl cyclase , g protein , gtp' , gs alpha subunit , adcy9 , adcy10 , biochemistry , biology , adenylate cyclase toxin , pertussis toxin , cyclase , cholera toxin , gtp binding protein regulators , adenylate kinase , receptor , microbiology and biotechnology , enzyme
We have previously demonstrated the stimulation of adenylyl cyclase by guanine nucleotides in the plasma membranes of the aquatic fungus Blastocladiella emersonii . The existence of a G‐protein in these membranes similar to Gs, the G‐protein responsible for hormonal stimulation of adenylyl cyclase in mammals, was shown. In this report the crude membranes were further analyzed and a polypeptide of ~ 48 kDa reacted with the G α common GA /1 antibody, which recognises the GTP binding site of G‐proteins. A polypeptide of similar molecular mass is ADP‐ribosylated with pertussis toxin on a cysteine residue, like the counterparts in mammals (Gi, Gt and Go). The appearance of this Gi protein is developmentally controlled, as judged from pertussis toxin labelling and immunoblotting. We suggest the involvement of this G‐protein in the control of adenylyl cyclase activity, or ion channels, thus regulating the rapid transition between zoospores and vegetative cells.