Open AccessThe location and deletion of the genes which code for SSI‐like protease inhibitors in Streptomyces species
Author(s) -
Kuramoto Akinori,
Lezhava Alexander,
Taguchi Seiichi,
Momose Haruo,
Kinashi Haruyasu
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08176.x
Subject(s) - streptomyces coelicolor , gene , plasmid , biology , mutant , streptomyces , genetics , chromosome , dna , bacteria
The genes coding for the protease inhibitors, SSI and API‐2c′, have been analyzed by comparing DNA macrorestriction patterns of Streptomyces albogriseolus S‐3253 and S. griseoincamatus KTo‐250 with those of inhibitor‐deficient mutants. The mutants were found to suffer from chromosomal deletions rather than plasmid loss which resulted in the loss of the relevant genes. Hybridization experiments indicated that the ssi homologs in S. lividans and S. coelicolor A3(2) are located near the end of the linear chromosome.