Open AccessPhenotypic expression of a mannose‐sensitive hemagglutinin by a Vibrio cholerae O1 E1Tor strain and evaluation of its role in intestinal adherence and colonization
Author(s) -
Mukhopadhyay S,
Ghosh C,
Ghose A.C
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08162.x
Subject(s) - vibrio cholerae , microbiology and biotechnology , biology , mutant , hemagglutinin (influenza) , strain (injury) , serotype , el tor , hemagglutination , vibrionaceae , mannose , virology , bacteria , gene , genetics , virus , biochemistry , anatomy
A Vibrio cholerae O1 strain (1150) of the EITor biotype and Ogawa serotype with haemagglutination (HA) activity was subjected to TnphoA mutagenesis. Out of several mutants isolated, one HA − and another HA + mutant were further characterised. The HA − mutant showed about 50% reduction in its intestinal adherence capacity in vitro and about 9‐fold decrease of its colonisation ability in vivo, as compared to the wild‐type strain. Subsequent studies showed that the HA activity of strain 1150 was mediated by a mannose‐sensitive haemagglutinin (MSHA). Thus, the phenotypic expression of MSHA appears to be partly responsible for the intestinal adherence and colonisation properties of strain 1150.