Open AccessDetection of the single‐stranded DNA of Streptomyces plasmid pSA1.1 and a binding histone‐like protein
Author(s) -
Yokoyama Eiji,
Doi Katsumi,
Kimura Makoto,
Ogata Seiya
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08156.x
Subject(s) - plasmid , biology , dna , dna replication , rolling circle replication , microbiology and biotechnology , biochemistry , histone , plasmid preparation , pbr322
Streptomyces plasmid pSA1.1 accumulated single‐stranded DNA as replication intermediates in S. lividans ; therefore, this plasmid was considered to replicate by a rolling‐circle mechanism. A DNA‐binding protein (pI > 9.7 and about 10 kDa) was purified on a denatured DNA‐Cellulose column, then on a native DNA‐Cellulose column. The N‐terminal amino acid sequence of this protein has a high homology with bacterial histone‐like proteins. In the gel retardation assay, this protein bound with the single‐stranded DNA of pSA1.1. We propose that this protein may participate in the replication of pSA1.1.