Open AccessIncorporation of 2‐methyl‐3‐hydroxybutyric acid into polyhydroxyalkanoic acids by axenic cultures in defined media
Author(s) -
Füchtenbusch Bernd,
Fabritius Dirk,
Steinbüchel Alexander
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08149.x
Subject(s) - axenic , chemistry , burkholderia , organic chemistry , mass spectrometry , biochemistry , chromatography , bacteria , biology , genetics
Alcaligenes eutrophus and Burkholderia cepacia synthesized and accumulated a terpolyester consisting of 3‐hydroxybutyric acid, 3‐hydroxyvaleric acid, and 2‐methyl‐3‐hydroxybutyric acid (2Me3HB) if the cells were cultivated in a mineral salts medium containing tiglic acid as the sole carbon source or in combination with gluconic acid. The presence of 1–2 mol% of 2Me3HB in the polyester was confirmed by comparison with chemically synthesized methyl ester of 2Me3HB and by nuclear magnetic resonance spectrometry as well as by gas chromatography/mass spectrometry. This is the first report of the incorporation of 2Me3HB by axenic cultures cultivated under defined conditions.