Open AccessIdentification of the gene encoding DNA topoisomerase I from Candida albicans
Author(s) -
Taylor Alison,
Giles Kellie,
Sarthy Aparna V,
McGonigal Tom,
Fostel Jennifer
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08143.x
Subject(s) - candida albicans , biology , gene , nucleic acid sequence , topoisomerase , saccharomyces cerevisiae , open reading frame , genetics , microbiology and biotechnology , gene product , primer (cosmetics) , dna , peptide sequence , gene expression , chemistry , organic chemistry
A gene encoding a type I topoisomerase (TOP1) was isolated from Candida albicans , sequenced, and expressed in Saccharomyces cerevisiae . The TOP1 gene was identified from a C. albicans genomic library by hybridization with the product of a polymerase chain reaction with degenerate primer sets encoding regions conserved in other TOP1 genes. A clone containing an open reading frame of 2463 bp and predicted to encode a protein of 778 amino acids with sequence similarity to eukaryotic type I topoisomerases was identified. The C. albicans TOP1 gene restored camptothecin sensitivity and increased the topoisomerase activity in S. cerevisiae , indicating that the DNA fragment encodes a functional C. albicans topoisomerase I.