Open AccessComparison of responses of DNA topoisomerase I from Candida albicans and human cells to four new agents which stimulate topoisomerase‐dependent DNA nicking
Author(s) -
Fostel J,
Montgomery D,
Lartey P
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08142.x
Subject(s) - topoisomerase , candida albicans , camptothecin , biology , topoisomerase inhibitor , dna , enzyme , biochemistry , topoisomerase iv , microbiology and biotechnology , gene , escherichia coli , dna gyrase
DNA topoisomerase I is a potential target for therapeutic antifungal agents predicted to have a fungicidal mode of action. This report describes four agents with varying degrees of selectivity for the fungal topoisomerase I compared to the human enzyme: 5‐hydroxy‐1H‐indole‐3‐acetic acid (5‐HIAA), quinizarin, dibenzo‐ p ‐dioxin‐2‐carboxylic acid and 7‐amino‐4‐hydroxy‐2‐naphthalenesulfonic acid. Taken together with the response of topoisomerase to camptothecin and aminocatechol, these data suggest that there are sufficient structural differences between the topoisomerase I from Candida albicans and human cells to allow selective targeting of the fungal topoisomerase I over its human counterpart.