Open AccessCloning of a Bacillus sp. BP‐23 gene encoding a xylanase with high activity against aryl xylosides
Author(s) -
Blanco Ana,
Díaz Pilar,
Martínez Josefina,
López Olga,
Soler Carolina,
Pastor F.I.Javier
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08120.x
Subject(s) - xylobiose , xylanase , escherichia coli , biochemistry , chemistry , enzyme , hydrolysis , aryl , xylan , cloning (programming) , glycoside hydrolase , gene , organic chemistry , alkyl , computer science , programming language
The xynB gene encoding a xylanase from the recently isolated Bacillus sp. strain BP‐23 has been cloned and expressed in Escherichia coli . The enzyme produced in this host shows a molecular size of 41 kDa and a pI of 4.5. The pH and temperature at which the highest activity was found were 5.5 and 50°C respectively. Crude xylanase B showed activity on xylan, aryl xylosides, xylotetraose and xylotriose, while xylobiose was not hydrolyzed by the enzyme. Xylanase B showed high specific activity on aryl xylosides, probably as a result of the transxylosidase activity detected.