Open AccessExamination of surface polysaccharides of Actinobacillus pleuropneumoniae serotype. 1 Grown under iron‐restricted conditions
Author(s) -
Paradis SoniaÉlaine,
Dubreuil Daniel,
Jacques Mario
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08106.x
Subject(s) - actinobacillus pleuropneumoniae , flow cytometry , serotype , monoclonal antibody , antigen , polysaccharide , strain (injury) , microbiology and biotechnology , biology , actinobacillus , chemistry , antibody , biochemistry , bacteria , immunology , genetics , anatomy
We investigated the expression of important Actinobacillus pleuropneumoniae surface polysaccharides, namely, capsular polysaccharides (CPS) and lipopolysaccharides (LPS), after growth under iron‐restricted conditions. Iron restriction did not seem to affect the production of CPS, as determined by labelling with a monoclonal antibody (mAb) against the serotype l K‐antigen and flow cytometry analysis, and also as determined by electron microscopy. SDS‐PAGE revealed that the LPS profiles of these cells were also unaffected by iron restriction. Using flow cytometry analysis, however, we observed that binding of mAb against serotype 1 O‐antigen was altered in cells of A. pleuropneumoniae serotype l reference strain (4074) grown under iron‐restricted conditions. This strain exhibited two subpopulations with distinct patterns of reactivity with the mAb against the O‐antigen. When strain 4074 was grown under iron‐restricted conditions, a shift from one cell subpopulation (moderately fluorescent) to another cell subpopulation (highly fluorescent, thus binding more antibodies) was observed. Our results indicate that growth of A. pleuropneumoniae serotype l under iron‐restricted conditions did not seem to affect CPS production, but might alter, at least for the reference strain, the expression of LPS.