Open AccessPromoter analysis of the catalase‐peroxidase gene ( cpeA ) from Rhodobacter capsulatus
Author(s) -
Forkl Hubert,
Drews Gerhart,
Tadros Monier Habib
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08101.x
Subject(s) - rhodobacter , primer extension , promoter , start codon , gene , microbiology and biotechnology , lac operon , transcription (linguistics) , biology , gene expression , genetics , messenger rna , mutant , linguistics , philosophy
The expression of the Rhodobacter capsulatus catalase‐peroxidase gene ( cpeA ) was studied by in‐frame fusions of the upstream region of the cpeA gene to a promoter‐less lacZ gene. The transcription of the cpeA gene is about 20‐50‐fold higher under aerobic‐dark than under anaerobic‐light conditions. The promoter was localized within a 69‐bp upstream DNA region. The transcription start site, determined by primer extension, is 28 bases upstream from the initiation codon, confirming the postulated promoter localized by deletion analysis. Deletion of the part of the upstream region specifically responsible for oxygen regulation resulted in constitutive expression of the cpeA gene.