Open AccessMolecular cloning of a novel allele of SMR 1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae
Author(s) -
Xie Qi,
Jiménez Antonio
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08100.x
Subject(s) - saccharomyces cerevisiae , genetics , allele , transformation (genetics) , biology , coding region , gene , yeast , cloning (programming) , mutation , molecular cloning , saccharomyces , peptide sequence , computer science , programming language
A different mutation ( SMR1B ) to SMR1‐410 (ilv2) , which determines resistance to sulfometuron methyl in Saccharomyces cerevisiae , was cloned by PCR. Sequence analysis indicated a C to T change at position 575 of the ILV2 coding sequence, which results in a proline transition to leucine at position 192. Similarly to SMR1‐410 , the SMR1B gene was confirmed as a convenient dominant selective marker for yeast transformation including industrial strains.