Open AccessBiological activities of lipopolysaccharide of Burkholderia (Pseudomonas) pseudomallei
Author(s) -
Matsuura Motohiro,
Kawahara Kazuyoshi,
Ezaki Takayuki,
Nakano Masayasu
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08086.x
Subject(s) - lipopolysaccharide , burkholderia pseudomallei , melioidosis , lipid a , microbiology and biotechnology , splenocyte , pseudomonas , nitric oxide , tumor necrosis factor alpha , burkholderia , chemistry , macrophage , biology , bacteria , biochemistry , immunology , in vitro , endocrinology , genetics
Endotoxic activities of lipopolysaccharide (LPS) isolated from Burkholderia (Pseudomonas) pseudomallei , a causative agent of melioidosis, were investigated. Compared to an enterobacterial LPS (SAE‐LPS), B. pseudomallei LPS (BP‐LPS) exhibited weaker pyrogenic activity in rabbits, lethal toxicity in galactosamine‐sensitized mice and murine macrophage activation, i.e. production of tumor necrosis factor, interleukin‐6 and nitric oxide. BP‐LPS, on the other hand, exhibited stronger mitogenic activity to murine splenocytes than SAE‐LPS; moreover, it stimulated even the splenocytes of LPS‐resistant C3H/HeJ mice. Unusual chemical structures in the acid‐stable inner core region attached to the lipid A moiety of BP‐LPS may be responsible for this strong mitogenic activity.