Open AccessMolecular analysis of the promoter region of the hexokinase 2 gene of Saccharomyces cerevisiae
Author(s) -
MartínezCampa Carlos,
Herrero Pilar,
Ramírez Mario,
Moreno Fernando
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08084.x
Subject(s) - hexokinase , psychological repression , gene , promoter , saccharomyces cerevisiae , regulatory sequence , biology , gene expression , coding region , transcription (linguistics) , regulation of gene expression , lac operon , yeast , microbiology and biotechnology , regulator gene , genetics , biochemistry , enzyme , glycolysis , linguistics , philosophy
lacZ fusions of the hexokinase 2 gene promoter were constructed and a deletion analysis was performed in order to identify the cis ‐acting regulatory elements of the promoter that controls hexokinase 2 gene expression. Expression of the hexokinase 2 gene is induced by glucose and around 40‐fold repressed by ethanol. This repression seems to be mediated mainly by a repression element located within the coding region of the hexokinase 2 gene, between + 39 and + 404 bp from the ATG start codon. A second repressing element for ethanol growing cells was located between −455 bp and −254 bp. A synergistic effect on repression of transcription, when ethanol is the carbon source used for growth, was demonstrated by experiments in which both repressing elements were simultaneously removed. The finding of regulatory sequences in the coding region of the hexokinase 2 gene stimulates a search for regulatory elements in the coding region of other yeast genes.