Open AccessIsolation and characterization of a mutant of Saccharomyces cerevisiae affected in the FLO1 locus
Author(s) -
Reboredo Natalia M.,
Sieiro Carmen,
Blanco Pilar,
Villa Tomás G.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08082.x
Subject(s) - locus (genetics) , saccharomyces cerevisiae , genetics , ploidy , mutant , biology , gene , complementation , phenotype
A non‐flocculent strain of Saccharomyces cerevisiae was selected after EMS mutation of a flocculent and heterozygous FLO1 locus diploid. The analysis of 25 asci from this diploid showed in all cases segregation 0F:4NF, thus confirming that it was probably affected in the desired gene. After sporulation and dissection of asci, three haploid strains were chosen, which were altered in the locus FLO1 . Crossing these three strains with two other ones having markers for ADE1 and pho11::LEU2 , we could map the mutation at ca. 4.3 cM and ca. 37.7 cM from the PHO11 and ADE1 loci respectively.