Open AccessA putative signal recognition particle receptor α subunit (SRα) homologue is expressed in the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius
Author(s) -
Moll Ralf,
Schmidtke Silke,
Schaefer Günter
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08081.x
Subject(s) - sulfolobus acidocaldarius , sulfolobus , biology , open reading frame , transcription (linguistics) , gene , nucleic acid sequence , protein subunit , consensus sequence , genetics , primer extension , microbiology and biotechnology , archaea , rna , peptide sequence , linguistics , philosophy
A 1.64 kb genomic DNA sequence from the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius is composed of two adjacent genes. The first functionally unassigned open reading frame (orf‐1) comprises 450 base pairs. The second 1.1 kb large open reading frame encodes the putative signal recognition particle receptor α subunit (SRα). Both genes are expressed under the heterotrophic growth conditions of the organism. The main transcript of orf‐1 appears as a monocistronic RNA in Northern hybridization. With regard to SRα the transcription pattern was investigated by reverse transcription polymerase chain reaction and primer extension analysis. A polyclonal antiserum directed against E. coli lacZ′/Sulfolobus SRα fusion protein detects a 40.5 kDa protein (p41) in agreement with the 41.4 kDa as deduced from the nucleotide sequence.