Open AccessStudy of the intergenic exeF‐exeG region and its application as a simple preliminary test for Aeromonas spp.
Author(s) -
Karlyshev Andrey V.,
Macintyre Sheila
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08079.x
Subject(s) - intergenic region , aeromonas , aeromonas caviae , biology , aeromonas salmonicida , polymerase chain reaction , aeromonas veronii , pcr test , genetics , microbiology and biotechnology , bacteria , genome , gene
The exeF‐exeG intergenic regions from different hybridization groups (HG) of Aeromonas were studied by PCR amplification using a single pair of primers. Six main classes of PCR products were identified according to size: 360 bp, 320 bp, 280 bp, 230–240 bp, 220 bp and 160 bp. Direct sequencing of the PCR products indicated that the shorter intergenic regions had probably originated from deletion of DNA segments between direct repeats. Correlation of certain PCR products with Aeromonas caviae (HG4), A. caviae (HG5), A. veronii (HG8) and A. salmonicida (HG3) was revealed. The PCR reaction was also shown to be generally specific for Aeromonas spp. Thus, the usefulness of this rapid, single colony‐based PCR test for both identification and preliminary differentiation of Aeromonas spp. is demonstrated.