Open AccessMutations in Bacillus subtilis PyrR, the pyr regulatory protein, with defects in regulation by pyrimidines
Author(s) -
Ghim SaYoul,
Switzer Robert L.
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08075.x
Subject(s) - operon , mutant , bacillus subtilis , biochemistry , biology , uracil , point mutation , pyrimidine metabolism , gene , rna , pyrimidine , mutation , genetics , chemistry , enzyme , bacteria , dna , purine
The pyrimidine nucleotide biosynthetic ( pyr ) operon in Bacillus subtilis is regulated by a transcriptional attenuation mechanism in which PyrR, a bifunctional pyr RNA‐binding attenuation protein/uracil phosphoribosyltransferase, plays a crucial role. A convenient procedure for isolation of pyrR mutants with defects in the regulation of pyr operon expression is described. The selection is based on the selection of spontaneous mutations that convert the pyrimidine‐sensitive growth of cpa strain (lacking arginine‐repressible carbamyl phosphate synthetase) to pyrimidine resistance. Twelve such mutants were isolated and sequenced. All resulted from point mutations in the pyrR gene.