Open AccessIsolation and characterization of a functional promoter from Nitrosomonas europaea
Author(s) -
Nejidat Ali,
Spector Omit,
Abeliovich Aharon
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08074.x
Subject(s) - nitrosomonas europaea , promoter , nitrosomonas , biology , primer extension , obligate , reporter gene , escherichia coli , gene , genetics , consensus sequence , microbiology and biotechnology , gene expression , peptide sequence , rna , botany , nitrite , ecology , nitrate
Abstract A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli , using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at −35 and −10 have low similarity to the −10 / −35 consensus sequence of known prokaryotic promoters.