Open AccessDirect induction of homozygous diploidization in the fission yeast Schizosaccharomyces pombe by pressure stress
Author(s) -
Hamada Kazuhiro,
Nakatomi Yasuo,
Osumi Masako,
Shimada Shoji
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08058.x
Subject(s) - schizosaccharomyces pombe , ploidy , hydrostatic pressure , biology , auxotrophy , mating type , mating of yeast , yeast , osmotic pressure , mating , locus (genetics) , genetics , microbiology and biotechnology , botany , saccharomyces cerevisiae , gene , physics , mutant , thermodynamics
Hydrostatic pressure stress and a dye plate method were first used to investigate the direct induction of homozygous diploids from the haploid yeast Schizosaccharomyces pombe . Above 100 MPa at 25 °C for 10 min, pressure stress greatly inactivated the haploid strains of JY1 (L972 h − ) JY3 (L975 h 90 ) and JY334 ( ade 6‐M216 leul h + ). At the same time, when pressure stressed cells of these strains at more than 100–200 MPa were spread on a dye plate, some pressure‐effected visible colonies were stained violet (variant colonies); the rest were stained pink, similar to colonies originating from haploid cells that were not pressure‐stressed. Based on the cell size, DNA content, crosses, and random spore analyses for the segregation of mating types or auxotrophic markers, variant cells originating from color changed colonies of JY1 after pressure stress were very stable and found to be homozygous diploid with an h− / h− genotype at the mating‐type locus. From these results we conclude that pressure stress in combination with a dye plate is a simple and useful method for direct induction of homozygous diploid cells with very high stability.