Open AccessCharacterization of a secondary uptake system for l‐glutamate in Corynebacterium glutamicum
Author(s) -
Burkovski Andreas,
Weil Brita,
Krämer Reinhard
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08044.x
Subject(s) - corynebacterium glutamicum , glutamate receptor , kinetics , chemistry , sodium , biochemistry , strain (injury) , transport system , glutamic acid , corynebacterium , ion transporter , amino acid , bacteria , biology , membrane , organic chemistry , physics , receptor , genetics , anatomy , quantum mechanics , transport engineering , engineering , gene
A new transport system for the uptake of l‐glutamate was characterized in Corynebacterium glutamicum strain Δ glu, in which the previously described binding protein‐dependent glutamate uptake system is not present. Kinetic characterization revealed a highly specific secondary transport system, dependent on sodium ions. Glutamate uptake showed Michaelis‐Menten kinetics, with a K m of 0.6 mM and a V max of 15 nmol min −1 (mg dw) −1 . For the co‐transported sodium ions, a relatively low K m of 3.3 mM was determined.