Open AccessCharacterization of iturin synthetase in the wild‐type Bacillus subtilis strain producing iturin and in an iturin deficient mutant
Author(s) -
Feignier Christine,
Besson Françoise,
Michel Georges
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08036.x
Subject(s) - bacillus subtilis , mutant , strain (injury) , enzyme , biochemistry , lipopeptide , bacillales , protein subunit , bacillaceae , amino acid , biology , wild type , chemistry , microbiology and biotechnology , bacteria , gene , genetics , anatomy
The multi‐enzyme system responsible for the biosynthesis of iturin, an antifungal lipopeptide of Bacillus subtilis , was partially purified by chromatography on different affigels. In the wild‐type strain, two subunits of the iturin synthetase (ITs and ITagp) were characterized: ITs activated only l‐Ser, one of the iturin amino acid components, and ITagp activated l‐Asn, d‐Asn, l‐Gln and l‐Pro, amino acids corresponding to a partial sequence of iturin. In an iturin deficient mutant, the activity of the ITagp subunit was modified.