Open AccessSpecific interaction of the protein‐D2 porin of Pseudomonas aeruginosa with antibiotics
Author(s) -
Ishii Junko,
Nakae Taiji
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08029.x
Subject(s) - porin , microbiology and biotechnology , pseudomonas aeruginosa , antibiotics , piperacillin , chemistry , bacterial outer membrane , ampicillin , biology , bacteria , biochemistry , escherichia coli , gene , genetics
The protein‐D2 porin of Pseudomonas aeruginosa is lacking in carbapenem or fluoroquinolone‐resistant strains and hence was thought to facilitate the diffusion of these antibiotics. We examined the effect of several antibiotics on the single channel conductivity of protein‐D2 in planar lipid bilayers and found that fluoroquinolones and carbapenems at concentrations of around 1 mM caused closure of the protein‐D2 channel. Tetracycline, ampicillin, piperacillin, and latamoxef did not exert any detectable effect on the protein‐D2 channel activity.