Open AccessCharacterization of pepR1 , a gene coding for a potential transcriptional regulator of Lactobacillus delbrueckii subsp. lactis DSM7290
Author(s) -
Stucky Klaus,
Schick Joachim,
Klein Jürgen Robert,
Henrich Bernhard,
Plapp Roland
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08026.x
Subject(s) - open reading frame , biology , gene , reporter gene , coding region , regulatory sequence , genetics , transcription (linguistics) , regulator gene , catabolite repression , nucleic acid sequence , lactobacillus , microbiology and biotechnology , peptide sequence , gene expression , bacteria , linguistics , philosophy , mutant
The gene designated pepR1 , encoding a potential transcription regulator of Lactobacillus delbrueckii subsp. lactis DSM7290, was identified by sequence similarity of an open reading frame located upstream of the prolidase pepQ orientated in opposite direction. pepQ and pepR1 coding regions are spaced by 152 nucleotides. Upstream of the ‐35 region of pepQ, a 14‐bp palindromic sequence, homologous to the catabolite responsive element, could be identified. The pepR1 gene has the potential to encode a protein of 333 amino acids with a calculated molecular mass of 36955 Da and a calculated p/ of 5.5. The deduced protein sequence shows significant identity to the catabolite control protein of Bacillus. Co‐expression in Escherichia coli was studied with the pepR1‐pepQ intergenic region fused to the promoterless β‐galactosidase reporter gene. The pepQ ‐β‐galactosidase hybrid displayed an enhanced expression in the presence of cloned pepR1 .