Open AccessPolynucleobacter necessarius , an obligate bacterial endosymbiont of the hypotrichous ciliate Euplotes aediculatus , is a member of the β‐subclass of Proteobacteria
Author(s) -
Springer Nina,
Amann Rudolf,
Ludwig Wolfgang,
Schleifer KarlHeinz,
Schmidt Helmut
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08010.x
Subject(s) - obligate , ciliate , proteobacteria , subclass , biology , genetics , bacteria , botany , antibody , 16s ribosomal rna
An almost full length 16S rRNA gene of the obligate bacterial endosymbiont Polynucleobacter necessarius was amplified using the polymerase chain reaction in combination with site‐specific primers. The amplified DNA was directly sequenced and compared with other bacterial 16S rRNA sequences. P. necessarius belongs to the β‐subclass of Proteobacteria and shows the closest relationship to Alcaligenes eutrophus, Burkholderia solanacearum , and B. pickettii . In situ hybridization with a specific oligonucleotide probe corroborated the assignment of the retrieved sequence to P. necessarius .