Open AccessThe inactivation of hexokinase activity does not prevent glucose repression in Candida utilis
Author(s) -
Espinel Alberto E,
GómezToribio Víctor,
Peinado JoséM
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb08009.x
Subject(s) - hexokinase , chemostat , psychological repression , biochemistry , fed batch culture , catabolite repression , saccharomyces cerevisiae , enzyme , dehydrogenase , biology , enzyme repression , yeast , chemistry , glycolysis , bacteria , fermentation , gene expression , genetics , gene , mutant
High hexokinase activity was not related to glucose repression in Candida utilis IGC 3092. The addition of Cibacron Blue 3G‐A to growing cells in batch culture led to a permanent in vivo hexokinase inactivation, decreased growth rate and inhibited alcohol dehydrogenase. Hexokinase inactivation up to 90% did not alleviate glucose repression of α‐glucosidase, as has been described for Saccharomyces cerevisiae and other yeasts. Moreover, when cells were physiologically derepressed by growing them in a chemostat at low glucose concentrations, the highest hexokinase activity was shown by the derepressed cells, and decreased as repression increased. Thus, in our strain of C. utilis , hexokinase activity was inversely proportional to glucose repression.