Open AccessComparison of cytosolic levels of calcium and G actin in diffuse and localised adherent Escherichia coli ‐infected HeLa cells
Author(s) -
Karmaker Shyamali,
Ghosh Chaudhuri Alok,
Ganguly Uma
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb07996.x
Subject(s) - hela , extracellular , cytochalasin b , cytochalasin d , intracellular , actin , microfilament , egta , biology , calcium , escherichia coli , microbiology and biotechnology , cytosol , cytoskeleton , chemistry , cell , biochemistry , enzyme , organic chemistry , gene
In the present study we compared the intracellular level of free calcium ([Ca 2+ ] i ) and monomeric (G)/total (G + F) actin ratio in HeLa cells infected with diffuse (DAEC) and localised adherent Escherichia coli (LAEC). The level of [Ca 2+ ] i was increased in both DAEC‐ and LAEC‐infected HeLa cells. However, studies with EGTA‐ and dantrolene‐treated cells and also suspension of cells in Ca 2+ ‐free buffer suggested that the rise of [Ca 2+ ] i in DAEC‐infected cells was due to the influx of Ca 2+ from extracellular medium, whereas Ca 2+ mobilisation from the intracellular stores was responsible for the enhancement of [Ca 2+ ] i in LAEC‐infected cells. It was also evident that the infection of HeLa cells with DAEC and LAEC caused alteration of G / G + F actin ratio as compared to that of control cells. The ratio was much lower in LAEC‐infected cells than that of DAEC‐infected ones. Moreover, cytochalasin B inhibited both DAEC and LAEC invasion to HeLa cells, suggesting further the role of microfilaments in the invasion process.