Open AccessA stable Escherichia coli ‐mycobacteria shuttle vector ‘pSO246’ in Mycobacterium bovis BCG
Author(s) -
Matsumoto Sohkichi,
Tamaki Mikio,
Yukitake Hideharu,
Matsuo Takemitu,
Naito Manko,
Teraoka Hiroshi,
Yamada Takeshi
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb07995.x
Subject(s) - shuttle vector , mycobacterium bovis , microbiology and biotechnology , mycobacterium fortuitum , escherichia coli , plasmid , mycobacterium , mycobacterium kansasii , recombinant dna , vector (molecular biology) , biology , escherichia , virology , bacteria , mycobacterium tuberculosis , tuberculosis , medicine , genetics , dna , gene , pathology
The most widely used plasmid vector system in mycobacteria is based on pAL5000 from Mycobacterium fortuitum . The derivatives of the pAL5000‐based shuttle vectors between Escherichia coli and mycobacteria, which we have utilized to secrete recombinant antigens, were generated. The stability of the vectors was assessed in Mycobacterium bovis BCG (BCG). The plasmid vector pSO246 was stable in BCG for at least 50 generations.