Open AccessPolyhydroxyalkanoate synthesis from alcohols during the growth of Paracoccus denitrificans
Author(s) -
Yamane Tsuneo,
Chen XiangFu,
Ueda Shunsaku
Publication year - 1996
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1996.tb07991.x
Subject(s) - polyhydroxyalkanoates , paracoccus denitrificans , polyester , methanol , chemistry , ethanol , substrate (aquarium) , biodegradation , copolyester , organic chemistry , bacteria , butanol , nuclear chemistry , propanol , polymer chemistry , biology , enzyme , genetics , ecology
Several alcohols were examined as substrates for the polyhydroxyalkanoate synthesis by Paracoccus denitrificans. The bacterium synthesized a homopolyester of poly(3‐hydroxybutyrate) from ethanol. When n ‐pentanol was used as growth substrate, homopolyester poly(3‐hydroxyvalerate) was synthesized, whereas copolyester poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) accumulated during bacterial growth on n ‐propanol. When alcohols were automatically fed as growth substrates, ethanol, n ‐propanol, and n ‐pentanol gave higher polyester content. Although poly(3‐hydroxybutyrate) was synthesized from methanol or n ‐butanol, its content was very low. Under nitrogen‐deficient conditions, polyester;content in cells increased, especially with ethanol, n ‐propanol, and n ‐pentanol. Using a mixture of two alcohols P. denitrificans could synthesize polyesters with varying relative ratios of 3‐hydroxybutyrate to 3‐hydroxyvalerate.